ACNC

…IMPETUS ANIMAL WELFARE LTD ABN 54 660 772 547, an ACNC registered Australian not-for-profit entity ("Company"). 2. MEDICAL ETHICS PTY LTD ACN 606 628 353 of Level 27, 101 Collins…

30/03/2026]. See the Terms and Conditions (https://onlinelibrary.wiley.com/terms-and-conditions) on Wiley Online Library for rules of use; OA articles are governed by the applicable Creative Commons License 17510813, 2020, 6, Downloaded from https://onlinelibrary.wiley.com/doi/10.1111/avj.12930. By National Health And Medical Research Council, Wiley Online Library on [30/03/2026]. See the Terms and Conditions (https://onlinelibrary.wiley.com/terms-and-conditions) on Wiley Online Library for rules of use; OA articles are governed by the applicable Creative Commons License 17510813, 2020, 6, Downloaded from https://onlinelibrary.wiley.com/doi/10.1111/avj.12930. By National Health And Medical Research Council, Wiley Online Library on [30/03/2026]. See the Terms and Conditions (https://onlinelibrary.wiley.com/terms-and-conditions) on Wiley Online Library for rules of use; OA articles are governed by the applicable Creative Commons License

30/03/2026]. See the Terms and Conditions (https://onlinelibrary.wiley.com/terms-and-conditions) on Wiley Online Library for rules of use; OA articles are governed by the applicable Creative Commons License 17510813, 2020, 6, Downloaded from https://onlinelibrary.wiley.com/doi/10.1111/avj.12930. By National Health And Medical Research Council, Wiley Online Library on [30/03/2026]. See the Terms and Conditions (https://onlinelibrary.wiley.com/terms-and-conditions) on Wiley Online Library for rules of use; OA articles are governed by the applicable Creative Commons License 17510813, 2020, 6, Downloaded from https://onlinelibrary.wiley.com/doi/10.1111/avj.12930. By National Health And Medical Research Council, Wiley Online Library on [30/03/2026]. See the Terms and Conditions (https://onlinelibrary.wiley.com/terms-and-conditions) on Wiley Online Library for rules of use; OA articles are governed by the applicable Creative Commons License

cent, G.; Polkinghorne, A.; Solfen® (Animal Ethics Pty Ltd, Melbourne, Australia), a topical local anaesthetic and antiseptic Gottardo, F. Efficacy of formulation, instilled to the wound during the procedure, to alleviate subsequent castration-related Intra-Operative Topical Wound pain experienced in piglets. We performed a large, blind, multi-centred trial comparing pain in piglets Anaesthesia to Mitigate Piglet castrated with or without Tri-Solfen® . Piglets treated with wound instillation of Tri-Solfen® , 30 s prior Castration Pain—A Large, to subsequent castration, showed significantly lower pain-induced motor and vocal responses during Multi-Centred Field Trial. Animals the procedure. Acute post-operative pain-related behaviours, evident in piglets in the first 30 min 2021, 11, 2763. https:// doi.org/10.3390/ani11102763 following castration, were also significantly reduced in treated piglets compared with untreated piglets. Using this method, Tri-Solfen® provides an effective on-farm method to mitigate acute Academic Editor: Alison Small castration-related pain in young piglets. Received: 4 August 2021 Abstract: Piglet castration results in acute pain and stress to the animal. There is a critical need for Accepted: 15 September 2021 effective on-farm methods of pain mitigation. Local anaesthesia using Tri-Solfen® (Animal Ethics Published: 22 September 2021 Pty Ltd., Melbourne, Australia), a topical local anaesthetic and antiseptic formulation instilled to the wound during surgery, is a newly evolving on-farm method to mitigate castration pain. To Publisher’s Note: MDPI stays neutral investigate the efficacy of Tri-Solfen® , instilled to the wound during the procedure, to alleviate with regard to jurisdictional claims in subsequent castration-related pain

RESEARCH ARTICLE Topical anaesthesia reduces sensitivity of castration wounds in neonatal piglets Sabrina Lomax1☯, Charissa Harris1☯, Peter A. Windsor1‡, Peter J. White2‡* 1 Faculty of Science, School of Life and Environmental Science, The University of Sydney, Camperdown, NSW, Australia, 2 Faculty of Science, School of Veterinary Science, The University of Sydney, Camperdown, NSW, Australia ☯ These authors contributed equally to this work. ‡ These authors also contributed equally to this work. a1111111111 * p.white@sydney.edu.au a1111111111 a1111111111 a1111111111 a1111111111 Abstract The aim of this study was to do determine the efficacy of the topical anaesthetic Tri-Solfen® in the amelioration of the pain of castration in piglets. The trial was conducted over a three day period, and blocked across six litters with 12 piglets treated on days one and two, and OPEN ACCESS 16 on day three. The piglets were randomly allocated by weight and litter to 1 of 4 treatment Citation: Lomax S, Harris C, Windsor PA, White PJ groups: (i) sham castration (SHAM; n = 10); (ii) surgical castration with no anaesthetic inter- (2017) Topical anaesthesia reduces sensitivity of castration wounds in neonatal piglets. PLoS ONE vention (CAST; n = 10); (iii) surgical castration with post-operative topical anaesthesia (TRI; 12(11): e0187988. https://doi.org/10.1371/journal. n = 10); (iv) surgical castration with a pre-operative intra-testicular lignocaine hydrochloride pone.0187988 injection (LIG; n = 10). Wound sensitivity testing involved von Frey monofilaments of weights Editor: Francesco Staffieri, University of Bari, 4g and 300g, and an 18 gauge needle, used to stimulate the wound and surrounding skin ITALY respectively, at various pre-determined sites. Neonatal piglets