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American Dairy Science Association

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Topical Application of Lidocaine and Bupivacaine to Disbudding Wounds in Dairy Calves: Safety, Toxicology and Wound Healing 46%
Meredith Sheil; Michael Chambers; Adam Polkinghorne; Brendan Sharpe · file · #64

ng 5× the recommended dose, with no clinically significant differences Polkinghorne, A.; Sharpe, B. Topical in measured parameters between placebo and Tri-Solfen® groups. No negative impacts on wound Application of Lidocaine and healing were noted. Conversely, lower levels of bacterial wound colonisation were evident, and there Bupivacaine to Disbudding Wounds was reduced incidence of abnormal wounds at days 11–12 in Tri-Solfen® -treated animals. in Dairy Calves: Safety, Toxicology and Wound Healing. Animals 2021, Abstract: Tri-Solfen® is a combination topical anaesthetic and antiseptic solution containing lidocaine, 11, 869. https://doi.org/10.3390/ ani11030869 bupivacaine, adrenaline and cetrimide. Applied to wounds, it is reported to reduce the pain experi- enced by calves following thermocautery disbudding. While lidocaine and bupivacaine are widely Academic Editor: Alison Small used in medicine, conflicting data exist on the impact of these compounds when applied directly to the surgical wound. To investigate the safety of Tri-Solfen® applied to thermocautery disbudding Received: 22 January 2021 wounds of calves, experiments were performed to measure (i) the safety of Tri-Solfen® (including Accepted: 16 March 2021 in overdose situations); and (ii) the impact of Tri-Solfen® application at recommended doses on Published: 18 March 2021 disbudding wound healing under field conditions. Haematological, biochemical and urinalysis parameters did not show clinically significant differences between placebo and Tri-Solfen® groups Publisher’s Note: MDPI stays neutral (1×, 3× and 5× dose). No adverse health impacts were reported. Histopathological analysis of with regard to jurisdictional claims in wounds noted a reduction in bacterial colonies in Tri-Solfen®

Topical Wound-care Products and Their Effects on Healing, Inflammatory Biomarkers, and Growth in Piglets Undergoing Castration 4%
Laya Kannan Silva Alves; Monique Danielle Pairis-Garcia; Juliana Bonin Ferreira; Victoria Rocha Merenda; Rubia Mitalli Tomacheski; Pedro Henrique Esteves Trindade; Christopher Siepker; Magdiel Lopez-Soriano · file · #22

Topical Wound-care Products and Their Effects on Healing, Inflammatory Biomarkers, and Growth in Piglets Undergoing Castration Laya Kannan Silva Alves Universidade de São Paulo Monique Danielle Pairis-Garcia North Carolina State University Juliana Bonin Ferreira North Carolina State University Victoria Rocha Merenda North Carolina State University Rubia Mitalli Tomacheski Washington State University Pedro Henrique Esteves Trindade Michigan State University Christopher Siepker Iowa State University Magdiel Lopez-Soriano University of Missouri Research Article Keywords: acute phase proteins, animal welfare, piglet mortality, thermography, weaning, wound healing Posted Date: October 12th, 2025 DOI: https://doi.org/10.21203/rs.3.rs-7722871/v1 License:   This work is licensed under a Creative Commons Attribution 4.0 International License. Read Full License Additional Declarations: No competing interests reported. Page 1/20 Abstract Surgical castration is a routine management procedure in swine production that raises welfare concerns due to pain, inflammation, and risk of post-procedure complications. Topical products are commonly applied to castration wounds, but their efficacy in promoting healing and reducing inflammation has not been systematically evaluated. This study investigated the efficacy of five commercially available topical protective products on wound healing, inflammatory responses, and growth performance in piglets undergoing surgical castration. One hundred and ninety piglets were assigned to one of six groups: Iodine, Oinkment®, PhytoCare®, Vetericyn®, Zinc Oxide, or intact controls (NoCast). Treatments were applied immediately after castration (D1). Body weights were recorded at baseline (D0) and at weaning. Blood samples were collected on days 0

Evaluating the efficacy of a topical anaesthetic formulation and ketoprofen, alone and in combination, on the pain sensitivity of dehorning wounds in Holstein-Friesian calves 3%
Crystal A. Espinoza; Dominique McCarthy; Peter J. White; Peter A. Windsor; Sabrina H. Lomax · file · #39

…Topical anaesthesia for dehorning in calves Animal Production Science G To obtain quality data it is vital that behaviour observations be digital dermatitis in dairy cattle. Journal of Dairy Science 96, unobtrusive…

Clinical interpretation of body language and behavioral modifications to recognize pain in domestic mammals 2%
Daniel Mota-Rojas; Alexandra L. Whittaker; Lydia Lanzoni; Cécile Bienboire-Frosini; Adriana Domínguez-Oliva; Alfonso Chay-Canul; Vivian Fischer; Ismael Hernández-Avalos; Andrea Bragaglio; Eleonora Nannoni; Adriana Olmos-Hernández; Arthur Fernandes Bettencourt; Patricia Mora-Medina; Julio Martínez-Burnes; Alejandro Casas-Alvarado; Temple Grandin · file · #9

ou G, Elcoso G, Bubeck J, Bach A. Effects of oral administration of acidogenic boluses at dry-off on performance and behavior of dairy cattle. J Dairy Sci. (2018) 103. Cui W, Liu M, Gu T, Zhao S, Yin G. Multi-dimensional evaluation of pain 101:11342–53. doi: 10.3168/jds.2018-15058 response in low day-age calves to two types of dehorning. Front Vet Sci. (2024) 11:1406576. doi: 10.3389/fvets.2024.1406576 79. Tucker CB, Jensen MB, de Passillé AM, Hänninen L, Rushen J. Invited review: lying time and the welfare of dairy cows. J Dairy Sci. (2021) 104:20–46. doi: 104. Faulkner P, Weary D. Reducing pain after dehorning in dairy calves. J Dairy Sci. 10.3168/jds.2019-18074 (2000) 83:2037–41. doi: 10.3168/jds.S0022-0302(00)75084-3 80. Rial C, Laplacette A, Caixeta L, Florentino C, Peña-Mosca F, Giordano JO. Metritis 105. Graf B, Senn M. Behavioural and physiological responses of calves to dehorning and clinical mastitis events in lactating dairy cows were associated with altered patterns by heat cauterization with or without local anaesthesia. Appl Anim Behav Sci. (1999) of rumination, physical activity, and lying behavior monitored by an ear-attached sensor. 62:153–71. doi: 10.1016/S0168-1591(98)00218-4 J Dairy Sci. (2023) 106:9345–65. doi: 10.3168/jds.2022-23157 106. Mota-Rojas D, Martínez-Burnes J, Napolitano F, Domínguez-Muñoz M, 81. Schreiner DA, Ruegg PL. Responses to tail docking in calves and heifers. J Dairy Guerrero-Legarreta I, Mora-Medina P, et al. Dystocia: factors affecting parturition in Sci. (2002) 85:3287–96. doi: 10.3168/jds.S0022-0302(02)74417-2 domestic animals. CABI Rev. (2020) 2020:1–16. doi: 10.1079/PAVSNNR202015013 82. Kroll LK, Grooms DL, Siegford JM, Schweihofer JP, Daigle CL, Metz K, et al. 107. Mota-Rojas D, Lezama-García K,

Clinical interpretation of body language and behavioral modifications to recognize pain in domestic mammals 2%
Daniel Mota-Rojas; Alexandra L. Whittaker; Lydia Lanzoni; Cécile Bienboire-Frosini; Adriana Domínguez-Oliva; Alfonso Chay-Canul; Vivian Fischer; Ismael Hernández-Avalos; Andrea Bragaglio; Eleonora Nannoni; Adriana Olmos-Hernández; Arthur Fernandes Bettencourt; Patricia Mora-Medina; Julio Martínez-Burnes; Alejandro Casas-Alvarado; Temple Grandin · file · #76

ou G, Elcoso G, Bubeck J, Bach A. Effects of oral administration of acidogenic boluses at dry-off on performance and behavior of dairy cattle. J Dairy Sci. (2018) 103. Cui W, Liu M, Gu T, Zhao S, Yin G. Multi-dimensional evaluation of pain 101:11342–53. doi: 10.3168/jds.2018-15058 response in low day-age calves to two types of dehorning. Front Vet Sci. (2024) 11:1406576. doi: 10.3389/fvets.2024.1406576 79. Tucker CB, Jensen MB, de Passillé AM, Hänninen L, Rushen J. Invited review: lying time and the welfare of dairy cows. J Dairy Sci. (2021) 104:20–46. doi: 104. Faulkner P, Weary D. Reducing pain after dehorning in dairy calves. J Dairy Sci. 10.3168/jds.2019-18074 (2000) 83:2037–41. doi: 10.3168/jds.S0022-0302(00)75084-3 80. Rial C, Laplacette A, Caixeta L, Florentino C, Peña-Mosca F, Giordano JO. Metritis 105. Graf B, Senn M. Behavioural and physiological responses of calves to dehorning and clinical mastitis events in lactating dairy cows were associated with altered patterns by heat cauterization with or without local anaesthesia. Appl Anim Behav Sci. (1999) of rumination, physical activity, and lying behavior monitored by an ear-attached sensor. 62:153–71. doi: 10.1016/S0168-1591(98)00218-4 J Dairy Sci. (2023) 106:9345–65. doi: 10.3168/jds.2022-23157 106. Mota-Rojas D, Martínez-Burnes J, Napolitano F, Domínguez-Muñoz M, 81. Schreiner DA, Ruegg PL. Responses to tail docking in calves and heifers. J Dairy Guerrero-Legarreta I, Mora-Medina P, et al. Dystocia: factors affecting parturition in Sci. (2002) 85:3287–96. doi: 10.3168/jds.S0022-0302(02)74417-2 domestic animals. CABI Rev. (2020) 2020:1–16. doi: 10.1079/PAVSNNR202015013 82. Kroll LK, Grooms DL, Siegford JM, Schweihofer JP, Daigle CL, Metz K, et al. 107. Mota-Rojas D, Lezama-García K,

Exploratory Survey on European Consumer and Stakeholder Attitudes towards Alternatives for Surgical Castration of Piglets 2%
Marijke Aluwé; Evert Heyrman; João M. Almeida; Jakub Babol; Gianni Battacone; Jaroslav Čítek; Maria Font i Furnols; Andriy Getya; Danijel Karolyi; Eliza Kostyra; Kevin Kress; Goran Kušec; Daniel Mörlein; Anastasia Semenova; Martin Škrlep; Todor Stoyanchev; Igor Tomašević; Liliana Tudoreanu; Maren Van Son; Sylwia Żakowska-Biemans; Galia Zamaratskaia; Alice Van den Broeke; Macarena Egea · file · #3

al University of Life and Environmental Sciences of Ukraine (NULES), Henerala Rodimtseva 19, 03041 Kyiv, Ukraine; getya@ukr.net 8 Department of Animal Science, Faculty of Agriculture (UNIZG), University of Zagreb, Svetosimunska cesta 25, 10 000 Zagreb, Croatia; dkarolyi@agr.hr 9 Institute of Human Nutrition Sciences, Warsaw University of Life Sciences (WULS-SGGW), ul. Nowoursynowska 159c, 02-787 Warsaw, Poland; eliza_kostyra@sggw.edu.pl (E.K.); sylwia_zakowska_biemans@sggw.edu.pl (S.Ż.-B.) 10 Department of Behavioral Physiology of Livestock, Institute of Animal Science, University of Hohenheim, Garbenstraße 17, 70599 Stuttgart, Germany; kress.kevin@uni-hohenheim.de 11 Department of Animal Production and Biotechnology, Faculty of Agrobiotechnical Sciences Osijek, University of Osijek, Vladimira Preloga 1, 31000 Osijek, Croatia; gkusec@fazos.hr 12 Department of Animal Sciences, University of Göttingen, Albrecht-Thaer-Weg 3, 37075 Göttingen, Germany; daniel.moerlein@uni-goettingen.de 13 V.M. Gorbatov Federal Research Center for Food Systems of Russian Academy of Sciences, 26, Talalikhina str., 109316 Moscow, Russia; a.semenova@fncps.ru 14 Agricultural Institute of Slovenia, Hacquetova ulica 17, SI-1000 Ljubljana, Slovenia; martin.skrlep@kis.si 15 Department of Food safety and control of foodstuffs animal origin, Faculty of Veterinary medicine, Trakia University, Students Campus 6000, 6000 Stara Zagora, Bulgaria; todor.stoyanchev@uni-sz.bg 16 Department of Animal Source Food Technology, Faculty of Agriculture, University of Belgrade, Nemanjina 6, 11080 Belgrade, Serbia; tbigor@agrif.bg.ac.rs 17 Interdisciplinary Laboratory for Research on Heavy Metals Accumulation in the Food Chain and Modeling, Veterinary Medicine, Faculty of Veterinary Medicine and University of

Exploratory Survey on European Consumer and Stakeholder Attitudes towards Alternatives for Surgical Castration of Piglets 2%
Marijke Aluwé; Evert Heyrman; João M. Almeida; Jakub Babol; Gianni Battacone; Jaroslav Čítek; Maria Font i Furnols; Andriy Getya; Danijel Karolyi; Eliza Kostyra; Kevin Kress; Goran Kušec; Daniel Mörlein; Anastasia Semenova; Martin Škrlep; Todor Stoyanchev; Igor Tomašević; Liliana Tudoreanu; Maren Van Son; Sylwia Żakowska-Biemans; Galia Zamaratskaia; Alice Van den Broeke; Macarena Egea · file · #70

al University of Life and Environmental Sciences of Ukraine (NULES), Henerala Rodimtseva 19, 03041 Kyiv, Ukraine; getya@ukr.net 8 Department of Animal Science, Faculty of Agriculture (UNIZG), University of Zagreb, Svetosimunska cesta 25, 10 000 Zagreb, Croatia; dkarolyi@agr.hr 9 Institute of Human Nutrition Sciences, Warsaw University of Life Sciences (WULS-SGGW), ul. Nowoursynowska 159c, 02-787 Warsaw, Poland; eliza_kostyra@sggw.edu.pl (E.K.); sylwia_zakowska_biemans@sggw.edu.pl (S.Ż.-B.) 10 Department of Behavioral Physiology of Livestock, Institute of Animal Science, University of Hohenheim, Garbenstraße 17, 70599 Stuttgart, Germany; kress.kevin@uni-hohenheim.de 11 Department of Animal Production and Biotechnology, Faculty of Agrobiotechnical Sciences Osijek, University of Osijek, Vladimira Preloga 1, 31000 Osijek, Croatia; gkusec@fazos.hr 12 Department of Animal Sciences, University of Göttingen, Albrecht-Thaer-Weg 3, 37075 Göttingen, Germany; daniel.moerlein@uni-goettingen.de 13 V.M. Gorbatov Federal Research Center for Food Systems of Russian Academy of Sciences, 26, Talalikhina str., 109316 Moscow, Russia; a.semenova@fncps.ru 14 Agricultural Institute of Slovenia, Hacquetova ulica 17, SI-1000 Ljubljana, Slovenia; martin.skrlep@kis.si 15 Department of Food safety and control of foodstuffs animal origin, Faculty of Veterinary medicine, Trakia University, Students Campus 6000, 6000 Stara Zagora, Bulgaria; todor.stoyanchev@uni-sz.bg 16 Department of Animal Source Food Technology, Faculty of Agriculture, University of Belgrade, Nemanjina 6, 11080 Belgrade, Serbia; tbigor@agrif.bg.ac.rs 17 Interdisciplinary Laboratory for Research on Heavy Metals Accumulation in the Food Chain and Modeling, Veterinary Medicine, Faculty of Veterinary Medicine and University of

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