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Andre Dereu

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Nociception-Induced Changes in Electroencephalographic Activity and FOS Protein Expression in Piglets Undergoing Castration under Isoflurane Anaesthesia 11%
Judith Reiser; Matthias Kreuzer; Julia Werner; Anna M. Saller; Johannes Fischer; Steffanie Senf; Pauline Deffner; Nora Abendschön; Tanja Groll; Andrea Grott; Regina Miller; Shana Bergmann; Michael H. Erhard; Mathias Ritzmann; Susanne Zöls; Gerhard Schneider; Katja Steiger; Christine Baumgartner · file · #96

e FOS FOS staining staining intensity and frequency. (B) Area evaluated in the spinal cord. (C) Examples of different scores. intensity and frequency. (B) Area evaluated in the spinal cord. (C) Examples of different scores. The analysis 2.3. Statistical method was first established in a preliminary study (data not shown) to Analysis develop and define a scoring approach for formalin-fixed, paraffin-embedded and stained 2.3.1. EEG tissue sections. The staining intensity (none, slight, moderate, or strong) and the percentage The power of positive spectral neuronal density nuclei in bothof dorsal the selected horns EEG of theepisodes was calculated spinal cords with the were calculated. A MATLAB semiquantitative score including these two parameters (Figure 1A) was applied. from pwelch function and a frequency resolution of 0.25 Hz for the pooled data Four the interdigital different segmentspinch stimulus (L1, and 1S1-3) L2, L3, and Hz for the data in each recorded animal were evaluated. to other in responseThe resultsstimuli. of this Because evaluationof the larger clearly sample showed thesize in thescores highest pooledingroup for thecastrated the animals withoutwe pinch stimulus, decided pain relief. to useitawas Thus, higher frequency decided resolution to proceed to methodological with this better describe approach. the response. Density spectral arrays were also constructed, and the results are presented as heatmaps to depict the temporal evolution of the spectral EEG features. The DSA was derived from 5-s EEG episodes overlapping by 4 s. The results are presented as changes in the EEG power relative to a prestimulus baseline defined as the episode 50–40 s before the stimulus. The poststimulus value was divided by the prestimulus value. Thus, a value <1 indicates

Nociception-Induced Changes in Electroencephalographic Activity and FOS Protein Expression in Piglets Undergoing Castration under Isoflurane Anaesthesia 11%
Judith Reiser; Matthias Kreuzer; Julia Werner; Anna M. Saller; Johannes Fischer; Steffanie Senf; Pauline Deffner; Nora Abendschön; Tanja Groll; Andrea Grott; Regina Miller; Shana Bergmann; Michael H. Erhard; Mathias Ritzmann; Susanne Zöls; Gerhard Schneider; Katja Steiger; Christine Baumgartner · file · #29

e FOS FOS staining staining intensity and frequency. (B) Area evaluated in the spinal cord. (C) Examples of different scores. intensity and frequency. (B) Area evaluated in the spinal cord. (C) Examples of different scores. The analysis 2.3. Statistical method was first established in a preliminary study (data not shown) to Analysis develop and define a scoring approach for formalin-fixed, paraffin-embedded and stained 2.3.1. EEG tissue sections. The staining intensity (none, slight, moderate, or strong) and the percentage The power of positive spectral neuronal density nuclei in bothof dorsal the selected horns EEG of theepisodes was calculated spinal cords with the were calculated. A MATLAB semiquantitative score including these two parameters (Figure 1A) was applied. from pwelch function and a frequency resolution of 0.25 Hz for the pooled data Four the interdigital different segmentspinch stimulus (L1, and 1S1-3) L2, L3, and Hz for the data in each recorded animal were evaluated. to other in responseThe resultsstimuli. of this Because evaluationof the larger clearly sample showed thesize in thescores highest pooledingroup for thecastrated the animals withoutwe pinch stimulus, decided pain relief. to useitawas Thus, higher frequency decided resolution to proceed to methodological with this better describe approach. the response. Density spectral arrays were also constructed, and the results are presented as heatmaps to depict the temporal evolution of the spectral EEG features. The DSA was derived from 5-s EEG episodes overlapping by 4 s. The results are presented as changes in the EEG power relative to a prestimulus baseline defined as the episode 50–40 s before the stimulus. The poststimulus value was divided by the prestimulus value. Thus, a value <1 indicates

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