Invetus

study was funded by Invetus (grant line disease can be insidious, the disease was mild record 2018002127). and the horse responded well to supportive manage- ment. Any possible impact on wound healing…

…Ltd., Yarra Glen 3775, Australia 2 Invetus Pty. Ltd., Armidale 2350, Australia; mchambers@invetus.com (M.C.); bsharpe@invetus.com (B.S.) 3 Department of Microbiology and Infectious Diseases, NSW Health Pathology…

…Effects of a topically applied anaes- Invetus Pty Ltd, Armidale, NSW 2350, Timothy Pearce, Cert. Audio thetic on the behaviour, pain sensitivity and weight gain of dairy calves fol- Eng. (Sound Analysis…

CONSULTANCY DEED PARTIES 1. IMPETUS ANIMAL WELFARE LTD ABN 54 660 772 547, an ACNC registered Australian not-for-profit entity ("Company"). 2. MEDICAL ETHICS PTY LTD ACN 606 628 353 of Level 27, 101 Collins Street, Melbourne Victoria 3122 ("Client"). RECITALS A. The Client has agreed to engage the Company and the Company has agreed to accept the appointment to provide the Services to the Client on the terms and conditions of this Deed. B. The Company has agreed to perform certain Services according to the terms and conditions of this Deed. OPERATIVE PROVISIONS 1 DEFINITIONS AND INTERPRETATION 1.1 Definitions In this Deed, unless the context requires otherwise: "Commencement Date" means the date set out in Item 3 of Schedule 1; "Client" means Medical Ethics Pty Ltd (ACN 606 628 353) and its subsidiaries and related companies; "Company" means Impetus Animal Welfare Ltd, an ACNC registered Australian not- for-profit entity; "Confidential Information" means information and material relating to either party which comes into the possession or knowledge of another party, whether orally, in writing or electronically, directly or indirectly, including strategic, corporate and financial information of either party and information relating to the business, customers, sales, marketing plans, intellectual property, know-how and operations of either party; "Fees" means the fees payable to the Company as set out in Schedule 3 or other amount which may be determined and agreed by the parties in writing from time to time; "GST" means the goods and services tax payable under the GST Legislation; "GST Legislation" means A New Tax System (Goods and Services Tax) Act 1999 and related legislation passed by the Federal Government; "Indication" means a specific animal welfare use-case or

roduct that improves wound healing rates and decreases infection associated with castration is critical. Therefore, the objective of this study was to evaluate the efficacy of five topical protective products on wound healing, physiological response, inflammation, and performance in castrated piglets. MATERIAL AND METHODS Housing and animals This study was approved by the Institutional Animal Care and Use Committee of North Carolina State University (IACUC protocol 20–113). The experiment was conducted on a commercial sow farm in the southeastern United States during the summer. Sows and piglets were housed in individual farrowing crates within tunnel-ventilated, fully slatted farrowing rooms maintained at an average temperature of 22º ± 1.0 ºC. Temperature and ventilation were controlled using a computerized system. Each farrowing crate measured 2.5 m × 0.7 m, with an additional piglet area (2.5 m × 1.3 m). Heat mats were provided for piglets and maintained at approximately 30–35°C. Lighting was provided from 0600 to 1700 h. Feed and water were offered ad libitum to both sows and piglets. Page 3/20 Animal care and handling followed the Guide for the Care and Use of Agricultural Animals in Research and Teaching (FASS, 2020). Surgical castration was a routine farm practice; therefore, male piglets enrolled in this study were not castrated solely for research purposes. Experimental design and treatment administration A total of 190 Large White x Duroc male piglets from 51 litters were enrolled in this study (study duration = 21 days; Fig. 1). At enrollment (D0), piglets were individually identified using ear tags (Allflex Global Piglet ear tags, Allflex Livestock Intelligence, Madison, WI), weighed, and randomly assigned to one of six treatment groups: I

ticle. sodium chlorideFor(NaCl) the injection an automatic according to the studyself–filling system0.5 group. Then, 1 mL mL syringe ECO–MATIC® , (HSW intratesticularly were injected Henke-Sass, and Wolf GmbH,for 0.5 mL subscrotally Tuttlingen, Germany) each testicle. For the with a 25 Gan injection sized (0.5 × 16 mm, cannulaself–filling automatic B. Braun system 1 mL TravaCare GmbH, Hallbergmoos, Germany) was used. The intratesticular syringe (HSW ECO–MATIC , Henke-Sass, Wolf GmbH, Tuttlingen, Germany) with a 25 G sized ® injection was performed by cannula testicle fixing the(0.5 × 16between mm, B. theBraun thumbTravaCare and index finger GmbH, caudally. Hallbergmoos, Germany)the Directly following wasintratesticular used. The injection the subscrotal intratesticular injection single point injection was performed was performed by fixing the testiclebybetween releasing thethumb the fixed testicle, and indexretracting finger the cannula from it but leaving the cannula under the skin and making a skin caudally. Directly following the intratesticular injection the subscrotal single point injection was fold of the scrotum. The intratesticular performed and subscrotal by releasing injectionretracting the fixed testicle, was repeated for the from the cannula second testicle. it but Thereafter, leaving the piglets the cannula under wereskin the returned into the afarrowing and making skin foldpen for ascrotum. of the period ofThe 20 min. intratesticular and subscrotal injection was Afterwards, repeated all piglets for the second wereThereafter, testicle. removed again from were the piglets the farrowing returned peninto and, with the exception the farrowing pen for a of group period of H 20 (only min. fixation and simulated interventions), castrated. Therefore,

ticle. sodium chlorideFor(NaCl) the injection an automatic according to the studyself–filling system0.5 group. Then, 1 mL mL syringe ECO–MATIC® , (HSW intratesticularly were injected Henke-Sass, and Wolf GmbH,for 0.5 mL subscrotally Tuttlingen, Germany) each testicle. For the with a 25 Gan injection sized (0.5 × 16 mm, cannulaself–filling automatic B. Braun system 1 mL TravaCare GmbH, Hallbergmoos, Germany) was used. The intratesticular syringe (HSW ECO–MATIC , Henke-Sass, Wolf GmbH, Tuttlingen, Germany) with a 25 G sized ® injection was performed by cannula testicle fixing the(0.5 × 16between mm, B. theBraun thumbTravaCare and index finger GmbH, caudally. Hallbergmoos, Germany)the Directly following wasintratesticular used. The injection the subscrotal intratesticular injection single point injection was performed was performed by fixing the testiclebybetween releasing thethumb the fixed testicle, and indexretracting finger the cannula from it but leaving the cannula under the skin and making a skin caudally. Directly following the intratesticular injection the subscrotal single point injection was fold of the scrotum. The intratesticular performed and subscrotal by releasing injectionretracting the fixed testicle, was repeated for the from the cannula second testicle. it but Thereafter, leaving the piglets the cannula under wereskin the returned into the afarrowing and making skin foldpen for ascrotum. of the period ofThe 20 min. intratesticular and subscrotal injection was Afterwards, repeated all piglets for the second wereThereafter, testicle. removed again from were the piglets the farrowing returned peninto and, with the exception the farrowing pen for a of group period of H 20 (only min. fixation and simulated interventions), castrated. Therefore,

roduct that improves wound healing rates and decreases infection associated with castration is critical. Therefore, the objective of this study was to evaluate the efficacy of five topical protective products on wound healing, physiological response, inflammation, and performance in castrated piglets. MATERIAL AND METHODS Housing and animals This study was approved by the Institutional Animal Care and Use Committee of North Carolina State University (IACUC protocol 20–113). The experiment was conducted on a commercial sow farm in the southeastern United States during the summer. Sows and piglets were housed in individual farrowing crates within tunnel-ventilated, fully slatted farrowing rooms maintained at an average temperature of 22º ± 1.0 ºC. Temperature and ventilation were controlled using a computerized system. Each farrowing crate measured 2.5 m × 0.7 m, with an additional piglet area (2.5 m × 1.3 m). Heat mats were provided for piglets and maintained at approximately 30–35°C. Lighting was provided from 0600 to 1700 h. Feed and water were offered ad libitum to both sows and piglets. Page 3/20 Animal care and handling followed the Guide for the Care and Use of Agricultural Animals in Research and Teaching (FASS, 2020). Surgical castration was a routine farm practice; therefore, male piglets enrolled in this study were not castrated solely for research purposes. Experimental design and treatment administration A total of 190 Large White x Duroc male piglets from 51 litters were enrolled in this study (study duration = 21 days; Fig. 1). At enrollment (D0), piglets were individually identified using ear tags (Allflex Global Piglet ear tags, Allflex Livestock Intelligence, Madison, WI), weighed, and randomly assigned to one of six treatment groups: I

ry safety and efficacy field trials with the principles of Good Clinical Practice (GCP) as laid down in the Council Directive 2001/82/EC and guideline CVMP/VICH/595/98 VICH Topic GL9 Step 7-Guideline on Good Clinical Practices (CVMP approved July 2000). The animal study was performed, with owner consent, at two commercial pig farms in Germany and Italy dedicated to the management of breeding sows. Together, 173 commercially bred male piglets (Danish and Landrace ×Large White) between 3 and 7 days of age and in good health were confirmed suitable for enrolment in the study. Prior power analysis indicated the requirement for a minimum of 80 animals per group to show a significant reduction at the 5% level. Litters with 6–12 male piglets (7 average) were selected for the study at each site. Piglets within a litter were individually identified by ear tag (applied on study Day −2) and spray/pen mark. Piglets within a litter were randomly allocated to treatment or control groups. All study piglets had standard blue spray-on piglet marker dye applied to the scrotum prior to procedures, to blind post-operative observers to any translucent-blue colouring that may have remained following application of treatment. This method of blinding was used rather than a placebo solution, to allow comparison with standard castration practice, and prevent confounding due to potential impacts (pain/inflammation/infection) from wound instillation of a placebo solution. Hence, the person performing the treatment was unblinded, however, this person did not participate in any trial assessments or data analysis. Study animals were maintained in their normal farrowing pens as per standard farm practice with their dam and litter mates. Piglets had ad lib access to potable water and con- stant