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30/03/2026]. See the Terms and Conditions (https://onlinelibrary.wiley.com/terms-and-conditions) on Wiley Online Library for rules of use; OA articles are governed by the applicable Creative Commons License 17510813, 2020, 6, Downloaded from https://onlinelibrary.wiley.com/doi/10.1111/avj.12930. By National Health And Medical Research Council, Wiley Online Library on [30/03/2026]. See the Terms and Conditions (https://onlinelibrary.wiley.com/terms-and-conditions) on Wiley Online Library for rules of use; OA articles are governed by the applicable Creative Commons License 17510813, 2020, 6, Downloaded from https://onlinelibrary.wiley.com/doi/10.1111/avj.12930. By National Health And Medical Research Council, Wiley Online Library on [30/03/2026]. See the Terms and Conditions (https://onlinelibrary.wiley.com/terms-and-conditions) on Wiley Online Library for rules of use; OA articles are governed by the applicable Creative Commons License
30/03/2026]. See the Terms and Conditions (https://onlinelibrary.wiley.com/terms-and-conditions) on Wiley Online Library for rules of use; OA articles are governed by the applicable Creative Commons License 17510813, 2020, 6, Downloaded from https://onlinelibrary.wiley.com/doi/10.1111/avj.12930. By National Health And Medical Research Council, Wiley Online Library on [30/03/2026]. See the Terms and Conditions (https://onlinelibrary.wiley.com/terms-and-conditions) on Wiley Online Library for rules of use; OA articles are governed by the applicable Creative Commons License 17510813, 2020, 6, Downloaded from https://onlinelibrary.wiley.com/doi/10.1111/avj.12930. By National Health And Medical Research Council, Wiley Online Library on [30/03/2026]. See the Terms and Conditions (https://onlinelibrary.wiley.com/terms-and-conditions) on Wiley Online Library for rules of use; OA articles are governed by the applicable Creative Commons License
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tor(s) and not of MDPI and/or the editor(s). MDPI and/or the editor(s) disclaim responsibility for any injury to people or property resulting from any ideas, methods, instructions or products referred to in the content.
ence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. © The Author(s) 2023 Scientific Reports | (2023) 13:21237 | https://doi.org/10.1038/s41598-023-48551-1 10 Vol:.(1234567890)
ence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. © The Author(s) 2023 Scientific Reports | (2023) 13:21237 | https://doi.org/10.1038/s41598-023-48551-1 10 Vol:.(1234567890)
A, Fahey T. Predicting acute finisher pigs. Livest Sci. 2018;212:45–51. 0.1016/j.livsci.2018.03.013. uncomplicated urinary tract infection in women: a systematic review of the 153. Holinger M, Früh B, Stoll P, Kreuzer M, Hilmman E, editors. Fattening pigs dis- diagnostic accuracy of symptoms and signs. BMC Fam Pract. 2010;11:78. http play weak behavioural changes due to gastric ulcerations. Book of Abstracts s://doi.org/10.1186/1471-2296-11-78. of the 71st Annual Meeting of the European Federation of Animal Science, 176. Mehta P, Leslie SW, Reddivari AKR. Dysuria. StatPearls. Treasure Island (FL): 1st-4th December, 2020, Virtuel Meeting; 2020. StatPearls Publishing Copyright © 2023. StatPearls Publishing LLC.; 2023. 154. Zalecki M. The influence of antral ulcers on intramural gastric nerve projec- 177. Farmer C, Maes D, Peltoniemi O. Mammary system. In: Zimmermann JJ, tions supplying the pyloric sphincter in the pig (Sus scrofa domestica)—neu- Karriker, LA, Ramirez, A., Schwartz, K.J., Stevenson, GW, Zhang, J, editors. In: ronal tracing studies. PLoS ONE. 2015;10(5):e0126958. https://doi.org/10.1371 Diseases of Swine, ed.11th, pp. 313–338. https://doi.org/10.1002/9781119350 /journal.pone.0126958. 927.ch18 155. Zalecki M, Juranek J, Pidsudko Z, Mogielnicka-Brzozowska M, Kaleczyc J, 178. Chou JY, Marchant JN,
A, Fahey T. Predicting acute finisher pigs. Livest Sci. 2018;212:45–51. 0.1016/j.livsci.2018.03.013. uncomplicated urinary tract infection in women: a systematic review of the 153. Holinger M, Früh B, Stoll P, Kreuzer M, Hilmman E, editors. Fattening pigs dis- diagnostic accuracy of symptoms and signs. BMC Fam Pract. 2010;11:78. http play weak behavioural changes due to gastric ulcerations. Book of Abstracts s://doi.org/10.1186/1471-2296-11-78. of the 71st Annual Meeting of the European Federation of Animal Science, 176. Mehta P, Leslie SW, Reddivari AKR. Dysuria. StatPearls. Treasure Island (FL): 1st-4th December, 2020, Virtuel Meeting; 2020. StatPearls Publishing Copyright © 2023. StatPearls Publishing LLC.; 2023. 154. Zalecki M. The influence of antral ulcers on intramural gastric nerve projec- 177. Farmer C, Maes D, Peltoniemi O. Mammary system. In: Zimmermann JJ, tions supplying the pyloric sphincter in the pig (Sus scrofa domestica)—neu- Karriker, LA, Ramirez, A., Schwartz, K.J., Stevenson, GW, Zhang, J, editors. In: ronal tracing studies. PLoS ONE. 2015;10(5):e0126958. https://doi.org/10.1371 Diseases of Swine, ed.11th, pp. 313–338. https://doi.org/10.1002/9781119350 /journal.pone.0126958. 927.ch18 155. Zalecki M, Juranek J, Pidsudko Z, Mogielnicka-Brzozowska M, Kaleczyc J, 178. Chou JY, Marchant JN,
oler and centrifuged (2,000 × g for 15 min at 4°C) within eight hours of collection to separate serum. The serum was then aliquoted into 1.5ml Axygen® microcentrifuge tubes (Axygen Scientific, Corning, NY) at − 80°C. The assays for the biomarker analysis were completed six to nine months post-collection. PGE2 concentrations were measured using a commercial enzyme-linked immunosorbent assay (ELISA; catalog No. 514531; Cayman Chemical) following the method described by Giorgi et al., (2011). Briefly, serum samples were purified by adding ice-cold acetone (4x the serum volume), followed by incubation at − 20° C for 30 minutes and centrifugation (3,000 x g for 5 minutes). The supernatant was transferred to a 13 x 100-mm glass tube, evaporated using a CentriVap concentrator (Labconco), and reconstituted to the original serum volume with kit buffer. An aliquot of the reconstituted sample was derivatized with adjusted kit components and the manufacturer’s protocol were followed. Samples were analyzed in duplicate, and absorbance was measured at 405 nm following 60 minutes of development (SpectraMax i3; Molecular Devices). The mean PGE2 concentration of a reference sample used for repeatability assessment was 12.42 pg/ml (range: 9.90–15.60 pg/mL), with an inter-assay coefficient of variation of 8.61% (Merenda et al., 2022). Haptoglobin concentrations were determined using the Tridelta Phase® Haptoglobin Assay (catalog No. TP-801; Tridelta Development Ltd., Maynooth, Ireland). The assay utilized Haemoglobin (reagent 1), Chromogen (reagent 2), calibrator/sample diluent, and calibrator (microplate method). A standard curve was generated for each assay using haptoglobin standards at 2.5, 1.25, 0.625, 0.312, and 0 mg/mL. The Page 5/20 assay procedure involved