…In Sardinia, boars are raised and butchered to produce charcuterie. These animals are castrated before slaughter as androstenone would otherwise taint the meat, rendering it unfit for human consumption. However, to date…
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…In Sardinia, boars are raised and butchered to produce charcuterie. These animals are castrated before slaughter as androstenone would otherwise taint the meat, rendering it unfit for human consumption. However, to date…
itek, J.; Candek-Potokar, M.; Djekic, I.; Getya, A.; Guerrero, L.; Ivanova, S.; Kusec, G.; Nakov, D.; et al. Attitudes and beliefs of eastern european consumers towards animal welfare. Animals 2020, 10, 17. [CrossRef] [PubMed] © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
itek, J.; Candek-Potokar, M.; Djekic, I.; Getya, A.; Guerrero, L.; Ivanova, S.; Kusec, G.; Nakov, D.; et al. Attitudes and beliefs of eastern european consumers towards animal welfare. Animals 2020, 10, 17. [CrossRef] [PubMed] © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
ence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. © The Author(s) 2023 Scientific Reports | (2023) 13:21237 | https://doi.org/10.1038/s41598-023-48551-1 10 Vol:.(1234567890)
ence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. © The Author(s) 2023 Scientific Reports | (2023) 13:21237 | https://doi.org/10.1038/s41598-023-48551-1 10 Vol:.(1234567890)
o revisit the topic. Livestock Science 230, 103837. DOI: 10.1016/j.livsci.2019.103837. Genetic Resources (2021) S1 International Congress on Sheep and Goats 60 Abstract book
oler and centrifuged (2,000 × g for 15 min at 4°C) within eight hours of collection to separate serum. The serum was then aliquoted into 1.5ml Axygen® microcentrifuge tubes (Axygen Scientific, Corning, NY) at − 80°C. The assays for the biomarker analysis were completed six to nine months post-collection. PGE2 concentrations were measured using a commercial enzyme-linked immunosorbent assay (ELISA; catalog No. 514531; Cayman Chemical) following the method described by Giorgi et al., (2011). Briefly, serum samples were purified by adding ice-cold acetone (4x the serum volume), followed by incubation at − 20° C for 30 minutes and centrifugation (3,000 x g for 5 minutes). The supernatant was transferred to a 13 x 100-mm glass tube, evaporated using a CentriVap concentrator (Labconco), and reconstituted to the original serum volume with kit buffer. An aliquot of the reconstituted sample was derivatized with adjusted kit components and the manufacturer’s protocol were followed. Samples were analyzed in duplicate, and absorbance was measured at 405 nm following 60 minutes of development (SpectraMax i3; Molecular Devices). The mean PGE2 concentration of a reference sample used for repeatability assessment was 12.42 pg/ml (range: 9.90–15.60 pg/mL), with an inter-assay coefficient of variation of 8.61% (Merenda et al., 2022). Haptoglobin concentrations were determined using the Tridelta Phase® Haptoglobin Assay (catalog No. TP-801; Tridelta Development Ltd., Maynooth, Ireland). The assay utilized Haemoglobin (reagent 1), Chromogen (reagent 2), calibrator/sample diluent, and calibrator (microplate method). A standard curve was generated for each assay using haptoglobin standards at 2.5, 1.25, 0.625, 0.312, and 0 mg/mL. The Page 5/20 assay procedure involved
oler and centrifuged (2,000 × g for 15 min at 4°C) within eight hours of collection to separate serum. The serum was then aliquoted into 1.5ml Axygen® microcentrifuge tubes (Axygen Scientific, Corning, NY) at − 80°C. The assays for the biomarker analysis were completed six to nine months post-collection. PGE2 concentrations were measured using a commercial enzyme-linked immunosorbent assay (ELISA; catalog No. 514531; Cayman Chemical) following the method described by Giorgi et al., (2011). Briefly, serum samples were purified by adding ice-cold acetone (4x the serum volume), followed by incubation at − 20° C for 30 minutes and centrifugation (3,000 x g for 5 minutes). The supernatant was transferred to a 13 x 100-mm glass tube, evaporated using a CentriVap concentrator (Labconco), and reconstituted to the original serum volume with kit buffer. An aliquot of the reconstituted sample was derivatized with adjusted kit components and the manufacturer’s protocol were followed. Samples were analyzed in duplicate, and absorbance was measured at 405 nm following 60 minutes of development (SpectraMax i3; Molecular Devices). The mean PGE2 concentration of a reference sample used for repeatability assessment was 12.42 pg/ml (range: 9.90–15.60 pg/mL), with an inter-assay coefficient of variation of 8.61% (Merenda et al., 2022). Haptoglobin concentrations were determined using the Tridelta Phase® Haptoglobin Assay (catalog No. TP-801; Tridelta Development Ltd., Maynooth, Ireland). The assay utilized Haemoglobin (reagent 1), Chromogen (reagent 2), calibrator/sample diluent, and calibrator (microplate method). A standard curve was generated for each assay using haptoglobin standards at 2.5, 1.25, 0.625, 0.312, and 0 mg/mL. The Page 5/20 assay procedure involved
RESEARCH ARTICLE Effect of Topically Applied Anaesthetic Formulation on the Sensitivity of Scoop Dehorning Wounds in Calves Dominique McCarthy*, Peter Andrew Windsor, Charissa Harris, Sabrina Lomax, Peter John White School of Life and Environmental Sciences, Faculty of Veterinary Science, University of Sydney, Sydney, NSW, Australia * dominique.mccarthy@sydney.edu.au a11111 Abstract The post-operative effects of three formulations of topical anaesthetic and a cornual nerve block on the sensitivity of scoop dehorning wounds in calves were compared in two trials. In OPEN ACCESS Trial 1, 21 female Holstein dairy calves aged 8 to 24 weeks were randomly allocated to two Citation: McCarthy D, Windsor PA, Harris C, Lomax groups: (1) scoop dehorning with a post-operative application of a novel topical anaesthetic S, White PJ (2016) Effect of Topically Applied powder (DTAP, n = 10); and (2) scoop dehorning with a post-operative application of a Anaesthetic Formulation on the Sensitivity of Scoop novel topical anaesthetic ethanol liquid (DTAE, n = 11). In Trial 2, 18 castrated male and 18 Dehorning Wounds in Calves. PLoS ONE 11(9): female Hereford beef calves aged 16 to 20 weeks were randomly allocated to four groups: e0163181. doi:10.1371/journal.pone.0163181 (1) scoop dehorning with a pre-operative cornual nerve block of lignocaine (DCB, n = 9); (2) Editor: Francesco Staffieri, University of Bari, ITALY scoop dehorning with a post-operative application of the novel topical anaesthetic ethanol Received: June 7, 2016 liquid from Trial 1 (DTAE, n = 9); (3) scoop dehorning with a post-operative application of a Accepted: September 2, 2016 topical anaesthetic gel (DTAG, n = 9); and (4) sham dehorning (CON, n = 9). Sensitivity was Published: September 20, 2016 assessed by scoring the