UTIs

…In the review, however, no studies tion revealed dermal and subcutaneous pyogranuloma- elaborating on pain in relation to UTI in pigs were found. tous lesions due to infection with Staphylococcus aureus This…

…on a decision of the Parliament of the Federal needed for rare diseases (such as UTIs) and topics that Republic of Germany, granted by the Federal Office for Agriculture and Food were…

e commer­ of orf virus. This was followed by a similar sampling of cial MagMAX™ Pathogen RNA/DNA kit (Thermo Fisher the lesions on days 1 (T1), 3 (T2) and 5 (T3) post- Scientific) and the automated magnetic particle processor treatment of all the lesions observed on the lambs. KingFisher Flex System (Thermo Fisher Scientific), fol­ Samples of 3mL of whole blood were also collected lowing the manufacturer’s instructions. Extracted DNA from the jugular vein through a vacutainer system into samples were stored at −80°C until end of sampling in EDTA tubes to perform haematology in all the animals order to evaluate all of them in a single run of real-time prior to (T0) and ten days (T4) following treatment, PCR quantification. Orf virus detection was performed For personal use only. respectively. using the commercial qPCR kit EXOone Contagious Ecthyma (Exopol, Spain) which targets the BL2 gene Treatment Application that encodes a major envelope viral antigen. The kit con­ Following confirmation of the presence of orf virus in the tains a quantified synthetic positive control, and also an lesions, a single spray of 1.5mL of TS was applied liber­ endogenous control to avoid false-negative results. ally with a spray gun to all orf lesions in Group A lambs, Amplification was performed on a FAST 7500 cycler with the Group B lambs remaining untreated. (Applied Biosystems), and a cut-off value for positive samples was established at cycle quantification (Cq) Clinical Progression values lower than 38. Clinical examination of all lambs was performed by obser­ vers blinded to treatment. This occurred daily for 11 days Virus Culture to determine the clinical progression of the lesions, with Sterile swabs collected at T0, T1, T2 and T3 were submitted data collected on the

Topical Wound-care Products and Their Effects on Healing, Inflammatory Biomarkers, and Growth in Piglets Undergoing Castration Laya Kannan Silva Alves Universidade de São Paulo Monique Danielle Pairis-Garcia North Carolina State University Juliana Bonin Ferreira North Carolina State University Victoria Rocha Merenda North Carolina State University Rubia Mitalli Tomacheski Washington State University Pedro Henrique Esteves Trindade Michigan State University Christopher Siepker Iowa State University Magdiel Lopez-Soriano University of Missouri Research Article Keywords: acute phase proteins, animal welfare, piglet mortality, thermography, weaning, wound healing Posted Date: October 12th, 2025 DOI: https://doi.org/10.21203/rs.3.rs-7722871/v1 License:   This work is licensed under a Creative Commons Attribution 4.0 International License. Read Full License Additional Declarations: No competing interests reported. Page 1/20 Abstract Surgical castration is a routine management procedure in swine production that raises welfare concerns due to pain, inflammation, and risk of post-procedure complications. Topical products are commonly applied to castration wounds, but their efficacy in promoting healing and reducing inflammation has not been systematically evaluated. This study investigated the efficacy of five commercially available topical protective products on wound healing, inflammatory responses, and growth performance in piglets undergoing surgical castration. One hundred and ninety piglets were assigned to one of six groups: Iodine, Oinkment®, PhytoCare®, Vetericyn®, Zinc Oxide, or intact controls (NoCast). Treatments were applied immediately after castration (D1). Body weights were recorded at baseline (D0) and at weaning. Blood samples were collected on days 0