University of Queensland

…University of Queensland, Gatton, Treatment was applied once daily for 7 days following wounding on day 0. Queensland, Australia. Mechanical thresholds were measured after treatment on days 1, 2 and 3. Email…

RESEARCH ARTICLE Effect of Topically Applied Anaesthetic Formulation on the Sensitivity of Scoop Dehorning Wounds in Calves Dominique McCarthy*, Peter Andrew Windsor, Charissa Harris, Sabrina Lomax, Peter John White School of Life and Environmental Sciences, Faculty of Veterinary Science, University of Sydney, Sydney, NSW, Australia * dominique.mccarthy@sydney.edu.au a11111 Abstract The post-operative effects of three formulations of topical anaesthetic and a cornual nerve block on the sensitivity of scoop dehorning wounds in calves were compared in two trials. In OPEN ACCESS Trial 1, 21 female Holstein dairy calves aged 8 to 24 weeks were randomly allocated to two Citation: McCarthy D, Windsor PA, Harris C, Lomax groups: (1) scoop dehorning with a post-operative application of a novel topical anaesthetic S, White PJ (2016) Effect of Topically Applied powder (DTAP, n = 10); and (2) scoop dehorning with a post-operative application of a Anaesthetic Formulation on the Sensitivity of Scoop novel topical anaesthetic ethanol liquid (DTAE, n = 11). In Trial 2, 18 castrated male and 18 Dehorning Wounds in Calves. PLoS ONE 11(9): female Hereford beef calves aged 16 to 20 weeks were randomly allocated to four groups: e0163181. doi:10.1371/journal.pone.0163181 (1) scoop dehorning with a pre-operative cornual nerve block of lignocaine (DCB, n = 9); (2) Editor: Francesco Staffieri, University of Bari, ITALY scoop dehorning with a post-operative application of the novel topical anaesthetic ethanol Received: June 7, 2016 liquid from Trial 1 (DTAE, n = 9); (3) scoop dehorning with a post-operative application of a Accepted: September 2, 2016 topical anaesthetic gel (DTAG, n = 9); and (4) sham dehorning (CON, n = 9). Sensitivity was Published: September 20, 2016 assessed by scoring the

RESEARCH ARTICLE Topical anaesthesia reduces sensitivity of castration wounds in neonatal piglets Sabrina Lomax1☯, Charissa Harris1☯, Peter A. Windsor1‡, Peter J. White2‡* 1 Faculty of Science, School of Life and Environmental Science, The University of Sydney, Camperdown, NSW, Australia, 2 Faculty of Science, School of Veterinary Science, The University of Sydney, Camperdown, NSW, Australia ☯ These authors contributed equally to this work. ‡ These authors also contributed equally to this work. a1111111111 * p.white@sydney.edu.au a1111111111 a1111111111 a1111111111 a1111111111 Abstract The aim of this study was to do determine the efficacy of the topical anaesthetic Tri-Solfen® in the amelioration of the pain of castration in piglets. The trial was conducted over a three day period, and blocked across six litters with 12 piglets treated on days one and two, and OPEN ACCESS 16 on day three. The piglets were randomly allocated by weight and litter to 1 of 4 treatment Citation: Lomax S, Harris C, Windsor PA, White PJ groups: (i) sham castration (SHAM; n = 10); (ii) surgical castration with no anaesthetic inter- (2017) Topical anaesthesia reduces sensitivity of castration wounds in neonatal piglets. PLoS ONE vention (CAST; n = 10); (iii) surgical castration with post-operative topical anaesthesia (TRI; 12(11): e0187988. https://doi.org/10.1371/journal. n = 10); (iv) surgical castration with a pre-operative intra-testicular lignocaine hydrochloride pone.0187988 injection (LIG; n = 10). Wound sensitivity testing involved von Frey monofilaments of weights Editor: Francesco Staffieri, University of Bari, 4g and 300g, and an 18 gauge needle, used to stimulate the wound and surrounding skin ITALY respectively, at various pre-determined sites. Neonatal piglets

al University of Life and Environmental Sciences of Ukraine (NULES), Henerala Rodimtseva 19, 03041 Kyiv, Ukraine; getya@ukr.net 8 Department of Animal Science, Faculty of Agriculture (UNIZG), University of Zagreb, Svetosimunska…

al University of Life and Environmental Sciences of Ukraine (NULES), Henerala Rodimtseva 19, 03041 Kyiv, Ukraine; getya@ukr.net 8 Department of Animal Science, Faculty of Agriculture (UNIZG), University of Zagreb, Svetosimunska…

cent, G.; Polkinghorne, A.; Solfen® (Animal Ethics Pty Ltd, Melbourne, Australia), a topical local anaesthetic and antiseptic Gottardo, F. Efficacy of formulation, instilled to the wound during the procedure, to alleviate subsequent castration-related Intra-Operative Topical Wound pain experienced in piglets. We performed a large, blind, multi-centred trial comparing pain in piglets Anaesthesia to Mitigate Piglet castrated with or without Tri-Solfen® . Piglets treated with wound instillation of Tri-Solfen® , 30 s prior Castration Pain—A Large, to subsequent castration, showed significantly lower pain-induced motor and vocal responses during Multi-Centred Field Trial. Animals the procedure. Acute post-operative pain-related behaviours, evident in piglets in the first 30 min 2021, 11, 2763. https:// doi.org/10.3390/ani11102763 following castration, were also significantly reduced in treated piglets compared with untreated piglets. Using this method, Tri-Solfen® provides an effective on-farm method to mitigate acute Academic Editor: Alison Small castration-related pain in young piglets. Received: 4 August 2021 Abstract: Piglet castration results in acute pain and stress to the animal. There is a critical need for Accepted: 15 September 2021 effective on-farm methods of pain mitigation. Local anaesthesia using Tri-Solfen® (Animal Ethics Published: 22 September 2021 Pty Ltd., Melbourne, Australia), a topical local anaesthetic and antiseptic formulation instilled to the wound during surgery, is a newly evolving on-farm method to mitigate castration pain. To Publisher’s Note: MDPI stays neutral investigate the efficacy of Tri-Solfen® , instilled to the wound during the procedure, to alleviate with regard to jurisdictional claims in subsequent castration-related pain

cent, G.; Polkinghorne, A.; Solfen® (Animal Ethics Pty Ltd, Melbourne, Australia), a topical local anaesthetic and antiseptic Gottardo, F. Efficacy of formulation, instilled to the wound during the procedure, to alleviate subsequent castration-related Intra-Operative Topical Wound pain experienced in piglets. We performed a large, blind, multi-centred trial comparing pain in piglets Anaesthesia to Mitigate Piglet castrated with or without Tri-Solfen® . Piglets treated with wound instillation of Tri-Solfen® , 30 s prior Castration Pain—A Large, to subsequent castration, showed significantly lower pain-induced motor and vocal responses during Multi-Centred Field Trial. Animals the procedure. Acute post-operative pain-related behaviours, evident in piglets in the first 30 min 2021, 11, 2763. https:// doi.org/10.3390/ani11102763 following castration, were also significantly reduced in treated piglets compared with untreated piglets. Using this method, Tri-Solfen® provides an effective on-farm method to mitigate acute Academic Editor: Alison Small castration-related pain in young piglets. Received: 4 August 2021 Abstract: Piglet castration results in acute pain and stress to the animal. There is a critical need for Accepted: 15 September 2021 effective on-farm methods of pain mitigation. Local anaesthesia using Tri-Solfen® (Animal Ethics Published: 22 September 2021 Pty Ltd., Melbourne, Australia), a topical local anaesthetic and antiseptic formulation instilled to the wound during surgery, is a newly evolving on-farm method to mitigate castration pain. To Publisher’s Note: MDPI stays neutral investigate the efficacy of Tri-Solfen® , instilled to the wound during the procedure, to alleviate with regard to jurisdictional claims in subsequent castration-related pain

Received: 15 September 2020 | Revised: 25 October 2020 | Accepted: 9 November 2020 DOI: 10.1111/tbed.13923 ORIGINAL ARTICLE Efficacy and application of a novel topical anaesthetic wound formulation for treating cattle with Foot-and-Mouth disease: A field trial in Cameroon Sevidzem S. Lendzele1,2 | Jacques F. Mavoungou1,3 | Kong A. Burinyuy4 | Koumba A. Armel1 | Simon J. Dickmu5 | James R. Young6 | Peter C. Thomson7 | Peter A. Windsor6 1 Institut de recherche en Ecologie Tropicale (IRET-CENAREST), Libreville, Gabon Abstract 2 Ecole Doctorale des Grandes Ecoles Recently, a wound dressing formulation, (Tri-Solfen®, Medical Ethics Pty Ltd, (EDGE), Libreville, Gabon Australia; TS) registered for use in ruminant husbandry in Australia, was registered 3 Université des Sciences et Techniques (USTM), Franceville, Gabon for Foot-and-Mouth Disease (FMD) therapy in large ruminants in Laos, following 4 School of Veterinary Science and Medicine, clinical observations of improved welfare and healing following treatment of FMD University of Ngaoundéré, Ngaoundéré, lesions. In November 2019, an FMD outbreak in Cameroon provided an opportunity Cameroon 5 for a field trial, comparing clinical responses and recoveries to treatments on a sam- The National Veterinary Laboratory (LANAVET), Garoua North, Cameroon ple of cattle (n = 36) comprising three equal groups of animals (n = 12), comparing 6 Sydney School of Veterinary Sciences, responses to three treatments: (i) the application to lesions of TS, (ii) the administra- The University of Sydney, Camden, NSW, Australia tion of parenteral oxytetraycline commonly used for FMD in Cameroon; and (iii) an 7 School of Life and Environmental Sciences, untreated control group (C). Appetite scores, lesion healing scores, and changes in The

TYPE Review PUBLISHED 15 October 2025 DOI 10.3389/fvets.2025.1679966 Clinical interpretation of body OPEN ACCESS language and behavioral modifications to recognize pain EDITED BY Melissa Bain, University of California…

TYPE Review PUBLISHED 15 October 2025 DOI 10.3389/fvets.2025.1679966 Clinical interpretation of body OPEN ACCESS language and behavioral modifications to recognize pain EDITED BY Melissa Bain, University of California…