NSW Health Pathology

…Ward 2 1 Ausvet Pty Ltd., Bruce, ACT, Australia, 2 Faculty of Science, Sydney School of Veterinary Science, University of Sydney, Camden, NSW, Australia, 3 South East Local Land Services, Goulburn, NSW…

Haemoglobin Concentration, White Blood Cells, Activated Partial Thromboplastin Time, Prothrombin Time and Fibrinogen) were observed. Group mean values for the key biochemical parameters (Alanine Aminotransferase, Albumin, Alkaline Phosphatase, Aspartate Aminotransferase, Creatinine, Creatine Ki- nase, Gamma-Glutamyltransferase, Globulin, Lactate Dehydrogenase, Total Protein and Urea) followed similar trends over time with any differences found between groups not statistically significant (Table S3). No divergence between parameters was observed at higher doses. Urine specimens from animals in each group were analysed for a range of key param- eters. A significant difference was noted for detectable blood (based on pseudoperoxidase activity of haemoglobin and myoglobin) between Group 4 (5× dose) and Group 1 (placebo) (p < 0.010). No significant differences were observed for any other parameters (Table 2). 3.1.3. Gross Pathology of Animals Following Euthanasia All animals were euthanised and subjected to necropsy at days 3 and 4 following disbudding and treatment. On gross examination at necropsy, lung consolidation was observed in 10/32 (31.3%) of animals, consistent with the presence of current or historical sub-clinical pneumonia. Of the 10 animals, 3 (30%) were from the placebo group, 2 (20%) were from Group 2, 3 (30%) were from Group 3 and 2 (20%) were from Group 4. No other lesions or abnormalities were observed with any other organ system. 3.1.4. Histopathological Analysis of Tissue Samples, Including Skin Histopathological analysis of tissue samples was undertaken on all Group 1 (placebo) and Group 4 (5× dose) animals. No evidence of pathology was noted in any organ sample examined. The microscopic findings from histopathological analysis of skin samples from

st known place of business of the party to be served; or (iii) the registered office of any party to be served, and in the case of posting such notice will be deemed to have been served 2 Business Days after such notice has been posted; or (c) if sent by email to the last known email address of the party to be served and will be deemed to have been served on the date of confirmed receipt. 14.2 Business Day A Business Day means a day other than a Saturday, Sunday, public holiday or bank holiday in Sydney and if something is to be done on a day which is not a Business Day then that thing must be done on the next or following Business Day. 14.3 Governing Law and Jurisdiction This Deed is governed by and must be construed in accordance with the laws of the State of New South Wales. The parties submit to the non-exclusive jurisdiction of the Courts of that State and the Commonwealth of Australia in respect of all matters or things arising out of this Deed. 14.4 Entire Deed This Deed contains the entire understanding between the parties concerning the subject matter of this Deed and supersedes all prior communications between the parties. Each party acknowledges that, except as expressly stated in this Deed, it has not relied on any representation, warranty or undertaking of any kind made by or on behalf of the other party in relation to the subject matter of this Deed. 14.5 No Assignment A party cannot assign or otherwise transfer the benefit of this Deed without the prior written consent of the other party. 14.6 No Subcontracting The Company must not subcontract the performance of the Services without the prior written consent of the Client. 14.7 No Variation This Deed cannot be amended or varied except in writing signed by the parties. 14.8 Counterparts If this Deed

…1,2 , David Guallar 1,2 and Marta Ruiz de Arcaute 1,2 1 Animal Pathology Department, Veterinary Faculty and Instituto Agroalimentario de Aragón-IA2 (Universidad de Zaragoza-CITA), Miguel Servet 177…

a 11 27.74±5.413b 24.4–32.5 fL B 3 31.50±8.150a 3 28.03±6.550b Mean corpuscular haemoglobin (MCH, pg) A 9 10.62±1.391a 11 9.664±1.097b 8.5–11.8 pg B 3 10.60±1.114a 3 9.533±1.168b Mean corpuscular haemoglobin concentration (MCHC, g/dL) A 9 22.04±2.559 11 35.28±2.734 32.3–42.0 g/dL B 3 34.50±5.112 3 34.63±4.332 For personal use only. Reticulocytes (K/µL) A 9 4.13±4.432 11 2.45±2.987 0.0–15.0 K/μL B 3 1.87±0.153 3 0.77±0.666 Leucocytes (WBC, K/µL) A 7 6.55±1.993a 10 11.06±7.86b 5.06–14.12 K/μL B 3 7.93±3.453a 3 9.17±4.788b Neutrophils (K/µL) A 7 3.66±1.575a 10 7.15±4.403b 1.17–6.11 K/μL B 3 4.83±2.695a 3 5.81±3.999b Lymphocytes (K/µL) A 7 2.73±0.960 10 3.39±1.037 2.54–9.6 K/μL B 3 2.51±0.933 3 2.41±0.925 Monocytes (K/µL) A 7 0.06±0.055a 10 0.32±0.289b 0.1–1.01 K/μL B 3 0.27±0.138a 3 0.90±0.638b Eosinophils (K/µL) A 7 0.01±0.007 10 0.08±0.111B 0.05–0.95 K/μL B 3 0.02±0.011 3 0.01±0.010A Basophil (K/µL) A 7 0.09±0.048 10 0.11±0.081 0–0.12 K/μL B 3 0.03±0.026 3 0.04±0.030 Platelets (K/µL) A 9 531.89±125.926 10 456.60±203.644 301–922 K/μL B 3 758.33±359.433 3 453.67±279.001 Notes: a,bDifferent letters mean significant differences between results in day one and day 11 (p<0.05); A,BDifferent letters mean significant differences between the two groups A and B (p<0.005). virus transmission during outbreaks. Similarly, hypocrellin shown to provide rapid and prolonged wound anaesthesia A antiviral activity seems to be related to a decline in from blockage of nociception,16 with the acidity poten­ pH.15 Low pH has been related to viricidal activity in tially sufficient to destroy virus without causing pain to the multiple models, including Herpes Simplex-1 (HSV-1) in animal. It has also been postulated

…6 Department of Microbiology and Infectious Diseases, NSW Health Pathology, Nepean Hospital, Penrith, NSW 2750, Australia; adam@majormitchellconsulting.com.au or adam.polkinghorne@health.nsw.gov.au 7 Major Mitchell Consulting, Buderim…

…6 Department of Microbiology and Infectious Diseases, NSW Health Pathology, Nepean Hospital, Penrith, NSW 2750, Australia; adam@majormitchellconsulting.com.au or adam.polkinghorne@health.nsw.gov.au 7 Major Mitchell Consulting, Buderim…

…6 Department of Microbiology and Infectious Diseases, NSW Health Pathology, Nepean Hospital, Penrith, NSW 2750, Australia; adam@majormitchellconsulting.com.au or adam.polkinghorne@health.nsw.gov.au 7 Major Mitchell Consulting, Buderim…

review of topical anaesthetics. Pediatr Emerg Care 1998;14: 419–423. (Accepted for publication 9 September 2012) BOOK REVIEW Color atlas of veterinary histology. 3rd edn. Edited by WJ Bacha and LM Bacha. Wiley-Blackwell, West Sussex, 2012. 356 pages. Price A$125.00. ISBN 978 0470958513. T he 3rd edition of this hardcover text covers histology in the is rudimentary and would not be of particular use to either students major veterinary species (dog, cat, horse, cow, sheep, goat, or pathologists as a reference. pig and chicken) and is designed as a learning tool for students. This edition is slightly longer and the layout of the text improved and more user friendly, especially the headings, compared with the The book begins with a brief introduction to histology, including 2nd edition, but the content is essentially unchanged. Helpful hints tips for viewing slides and setting up a microscope. All tissues and and root word boxes are a useful addition. Most of the illustrations systems are discussed chapter by chapter. There is a short introduc- from the 2nd edition are included, with some new ones. The CD is tion to each tissue or system, followed by annotated photomicro- new for the 3rd edition, but contains only the illustrations, which graphs relevant to the appropriate species. All photomicrographs are viewed via an index or search function. are in colour and diagrams are in black and white. The chapter on haematology is predominantly illustrated with blood smears rather than histological sections. It identifies the normal blood compo- The text is illustrative rather than an in-depth description of tissue. nents for each species and mentions common variations seen on Many of the medical histology books have such detailed analysis normal smears. The text concludes

…Ltd., Yarra Glen, 3775, Victoria, Australia; mlksheil@me.com 2 Department of Microbiology and Infectious Diseases, NSW Health Pathology, Nepean Hospital, Penrith, New South Wales, Australia; adam.polkinghorne@health.nsw.gov.au…